Fig. 5

Didymin inhibits apoptosis in PBCs. (A) Representative images of TUNEL staining in INS-1 cells. Scale bar = 100 μm. And quantification of the percentage of TUNEL positive cells (n = 3) (B) Apoptosis analysis of INS-1 cells by flow cytometry (n = 4). (C) Western blot analysis of the cleaved-PARP, PARP, Bax, Bcl2, cleaved-caspase3, and caspase3 proteins in INS-1 cells (n = 3). (D) Immunofluorescence staining of γ-H2AX (green) and insulin (red) in pancreas. Scale bar = 20 μm. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001 PA vs. PA + Didymin. # P < 0.05, ### P < 0.001, #### P < 0.0001 control vs. PA.