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Table 1 Primers’ sequences used in this study

From: The relevance of the non-invasive biomarkers lncRNA GAS5/miR-21 ceRNA regulatory network in the early identification of diabetes and diabetic nephropathy

LP and TG

BPS

AT (℃)

LP (bp)

β-actin(H)

F:5’ GTGGCCGAGGACTTTGATTG3’

R:5’ CCTGTAACAACGCATCTCATATT3’

60

73

lncRNA GAS5

F:5’GCAAGCCTAACTCAAGCCATT3’

R:5’CTCCACCATTTCAACTTCCAG3’

60

66

hsa-miR-191-5p

GSP:5’GGCAACGGAATCCCAAAAG3’

R: 5’GTGCGTGTCGTGGAGTCG3’

60

63

hsa-miR-21

GSP:5’GGGGGGTAGCTTATCAGACTG3’

R:5’CAGTGCGTGTCGTGGAGT3’

60

66

  1. Abbreviations: LP and TG: internal parameters and tested genes; BPS: bidirectional primer sequence; AT: annealing temperature; LP: length of products; GSP is a specific primer for the corresponding. miRNA and R are the primers that match the RT primer