Fig. 6

Effects of FAM129B and ADSCs-Exo on Nrf2 and HO-1 expression, pro-inflammatory cytokine secretion, and oxidative stress in podocytes treated with high glucose

(A) WB was used to detect the effects of ADSCs-Exo and high glucose treatment on FAM129B, VPS34, and iASPP expression in MPC5 cells, with GAPDH as an internal control. (B) WB was used to detect the effects of FAM129B siRNA and ADSCs-Exo on HO-1, Nrf2, and FAM129B expression in MPC5 cells treated with high glucose, with GAPDH as an internal control. (C) ELISA was used to detect the effect of FAM129B siRNA and ADSCs-Exo on the secretion of pro-inflammatory factors in MPC5 cells under high glucose treatment. (D) Flow cytometry was used to detect the effects of FAM129B siRNA and ADSCs-Exo on intracellular ROS content in MPC5 cells under high glucose treatment. (E) MDA kit was used to detect the effects of FAM129B siRNA and ADSCs-Exo on MDA content in MPC5 cells treated with high glucose. Data are presented as Mean ± SD and one-way analysis of variance was used to detect statistical differences between groups. Panel A, ns p > 0.05 VS. NG; **p < 0.01VS. NG; ##p < 0.01 VS. HG. Panel B, C, E, ** p < 0.01 VS. NG + si-NC; ns p > 0.05, ## p < 0.01 VS. HG + si-NC; ^^ p < 0.01 VS. HG + ADSCs-Exo + si-NC.