Fig. 3

Effect of HO-1 siRNA and ADSCs-Exo on the secretion of inflammatory cytokines and oxidative stress in podocytes under high glucose treatment

(A) WB was used to detect the effect of ADSCs-Exo on HO-1 expression in MPC5 cells under high glucose treatment, and GAPDH was used as an internal control. (B) WB was used to detect the effect of ADSCs-Exo on HO-1 expression in kidney tissue of model mice, and GAPDH was used as an internal control. (C) WB was used to detect the effect of HO-1 siRNA and ADSCs-Exo on HO-1 expression in MPC5 cells under high glucose treatment, with GAPDH as an internal control. (D) ELISA was used to detect the effects of HO-1 siRNA and ADSCs-Exo on the secretion of pro-inflammatory factors in MPC5 cells treated with high glucose. (E) Flow cytometry was used to detect the effects of HO-1 siRNA and ADSCs-Exo on ROS content in MPC5 cells under high glucose treatment. (F) The effect of HO-1 siRNA and ADSCs-Exo on MDA content of MPC5 cells treated with high glucose was detected by MDA kit. Data are presented as Mean ± SD and one-way analysis of variance was used to detect statistical differences between groups. Panel A, ns p > 0.05 VS. NG; ** p < 0.01 VS. NG; ## p < 0.01 VS. HG. Panel B, * p < 0.05 VS. db/m; ## p < 0.01 VS. db/db. Panel C, D, F, ** p < 0.01 VS. NG + si-NC; ns p < 0.05, ## p < 0.01 VS. HG + si-NC; ^^ p < 0.01 VS. HG + ADSCs-Exo + si-NC.