Fig. 4
From: LncRNA KCNQ1OT1 promotes the development of diabetic nephropathy by regulating miR-93-5p/ROCK2 axis
KCNQ1OT1 affected proliferation, apoptosis and fibrosis by sponging miR-93-5p HG-induced HGMC and HRGEC. HGMC and HRGEC were transfected with si-NC, si-KCNQ1OT1, si-KCNQ1OT1 + anti-miR-NC, or si-KCNQ1OT1 + anti-miR-93-5p and then treated with HG. A and B Cell proliferation was measured using CCK-8 assay. C Flow cytometry analysis was employed to determine apoptosis rate. D and E The protein levels of Bax and Bcl-2 were measured by western blot. F–I Western blot assay was performed to analyze the protein levels of FN, Col-I, Col-IV, E-Cadherin, vimentin, and N-Cadherin. *P < 0.05